| Irritancy to skin and eye | ||
 |
Acute irritancy to
skin to rabbits
1. Test purpose:
Determine if the tested pesticide irritates or erodes the mammal skin.
Estimate the possible similar harm when human touch the pesticide.
2. Materials and method:
2.1 test pesticide: Cymoxanil tech was provided by Saturn Agrochemical
Inc.
2.2 test animals: 4 white adult rabbits, half/sex with weight 2.0-2.2kg
were provided by Nanjing Medical University Animals Center. All the animals
were observed for one week before test, drank and fed freely. Environment
temperature of feeding animals was 20”Ą2”ę, humidity was 50%-80 % and it
was well ventilated. All rabbits were healthy without skin trauma.
2.3 test method:
All rabbits were removed hair for 2.5 ”Į 2.5 square centimeter each side
on the back 24h prior to test. Dissolve Cymoxanil with salad oil as per
1:1 (w/w). Take 0.5ml of Cymoxanil solution and daub on left side without
hair while right side was daubed with distilled water and used as contrast.
Cover with one layer of oilpaper and fix with gauze and rubberized fabric.
Wash off 4h later and observe and record skin irritant reaction in 1h,
24h and 48h.
3. The results:
According to the international criterion ”°intensity classification of
skin irritant”±, average integral of rabbit was 0. Cymoxanil tech was classified
as non-irritant to skin.
Results showed as follows:
| Sex |
1-hour |
24-hour |
48-hour irritant |
| intensity | e | ||
| erythema
edema |
erythema
edema |
rythema
edema |
|
Male |
0 0 |
0 0 |
0 0 |
| non-irritancy | |||
Male |
0 0 |
0 0 |
0 0 |
| non-irritancy | |||
Female |
0 0 |
0 0 |
0 0 |
| non-irritancy | |||
Female |
0 0 |
0 0 |
0 0 |
Average
integral |
0 |
0 |
0 |
Note: the results is only fit for the sample
Test: check: principal