Sensitizing effects on guinea-pigs

1.Test purpose:
Determine the abnormality reactivity (sensitizer) of Cymoxanil tech. when animals contact the pesticide repeatedly to produce immunity transfer’s possibility of skin reaction.

2. Materials and method:

2.1 test pesticide: Cymoxanil tech was provided by Saturn Agrochemical Inc.

2.2 test animals: 26g white healthy guinea-pigs with bodyweight 250-300g were provided by Nanjing provincial Laboratory Animals Center. All the animals were observed for one week before test, drank and fed freely. Environment temperature of feeding animals was 22±2℃, humidity was 40%-70% and it was well ventilated. All guinea-pigs were healthy without skin damnification.

3. Test method:
Use Buehler test method.
a) grouping: test animals were divided into two groups, including test group, and feminine contrast group. Remove hair on back 24h prior to daub.
b) Sensitizing touch: Remove hair for 3cm × 3cm area on the left side of each guinea-pig 24h prior to daub. Weigh 0.2g of Cymoxanil tech and dissolve in distilled water. Daub Cymoxanil solution on filter paper and adhibit the paper on guinea-pigs’ left side without hair. Cover with oilpaper and fix with rubberized fabric for 6h. Then, wash off and observe test results. Repeat at the 7th day and 14th day, respectively.
c) Inspiring touch: Remove hair for 2cm × 2cm on the right side of each guinea-pig. After sensitizing touch for 14d, weigh 0.1g of Cymoxanil tech and dissolve in distilled water, and daub the solution on unhair district. Cover with oilpaper and fix with rubberized fabric for 6h. Then, wash off. After inspiring touch, observe every day till the 12th day.

4. The results:
After 3 sensitizing touch, no adverse effects appeared on daubed pesticide district in test animals. And sensitizing ratio was 0%.
The results indicated that Cymoxanil was classified as not a skin sensitizer.

Note: the results is only fit for the sample